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KMID : 0545120080180061070
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Journal of Microbiology and Biotechnology
2008 Volume.18 No. 6 p.1070 ~ p.1075
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Asp97 is a Crucial Residue Involved in the Ligation of the [Fe4S4] Cluster of IscA from Acidithiobacillus ferrooxidans
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Jiang Huidan
Zhang Xiaojian
Ai Chenbin
Liu Yuandong
Zeng Jia
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Abstract
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IscA was proposed to be involved in the ironsulfur cluster assembly encoded by the iscSUA operon, but the role of IscA in the iron-sulfur cluster assembly still remains controversial. In our previous study, the IscA from A. ferrooxidans was successfully expressed in Escherichia coli, and purified to be a [Fe4S4]-cluster-containing protein. Cys35, Cys99, and Cys101 were important residues in ligating with the [Fe4S4] cluster. In this study, Asp97 was found to be another ligand for the iron-sulfur cluster binding according to sitedirected mutagenesis results. Molecular modeling for the IscA also showed that Asp97 was a strong ligand with the [Fe4S4] cluster, which was in good agreement with the experimental results. Thus, the [Fe4S4] cluster in IscA from A. ferrooxidans was ligated by three cysteine residues and one aspartic acid.
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KEYWORD
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